This experiment was conducted at the Poultry farm, College of Agriculture, University of Baghdad to determine the effect of feeding diets containing different levels of anise seeds or oil on reproductive performance of Lohmann roosters. A total of 30 Lohmann cocks, 24 weeks old were allocated at random into 5 treatment groups of 6 cocks each. Anise seeds and oil were added to the diet from the first day of experiment until the end of this experiment which lasted 8 months (July to March). Experimental treatments were as follows: Treatment 1 (C): control group, Treatment 2 (T1): supplementation of cocks ration with 2 kg anise seeds / Ton, Treatment 3 (T2): supplementation of cocks ration with 4 kg anise seeds / Ton, Treatment 4 (T3): supplementation of cocks ration wit 250 ml anise oil / Ton, and Treatment 5 (T4) : supplementation of cocks ration with 500 ml anise oil / Ton. Semen was collected from all cocks twice a month for evaluate semen traits which included semen volume, spermatozoa concentration, mass motility, individual motility, spermatocrit and percentages of dead, abnormal spermatozoa and acrosomal abnormalities. Semen Plasma traits were also evaluated in relation to concentrations of glucose, protein, cholesterol, and activities of alkaline phosphatase, GOT and GPT.
Results indicated that treated the cocks with anise seeds (T1 and T2) and anise oil (T3 and T4) resulted in significant increase (P<0.05) in semen volume, spermatozoa concentration, spermatozoa motility, spermatocrit and alkaline phosphatase activity in seminal plasma and significant decrease (P<0.05) in percentages of dead, abnormal spermatozoa and acrosomal abnormalities and concentrations of glucose, protein, cholesterol and activities of GOT and GPT enzymes in seminal plasma as compared with control group (C). However, T4 (500 ml anise oil / ton of diet) recorded the best results concerning all traits included in this experiment followed by the results of T3 (250 ml anise oil / ton of feed), whereas there were no significant differences between T1 (2 kg anise seeds / ton of diet) and T2 (4 kg anise seeds / ton of diet) with respect to all traits included in this study. On the other hand, results revealed that cocks recorded at general the best means for semen and seminal plasma traits during mild and cold months of the year (November, December, January and February) as compared with the hot months of the year included in this experiment (July, August, September and October). Furthermore, anise treatments (T1, T2, T3 and T4) surpass control group (C) as regards all characters included in this experiment during the hot period of year. In conclusion adding different levels of anise seeds and oil to the cocks' diet resulted in significant improvement in reproductive performance of the cocks reared during different months of the year. Furthermore anis oil treatments surpass anise seeds treatments as regards all semen and seminal plasma traits included in this experiment. Therefore, reproductive efficiency of roosters could be enhancing by supplementing diet of roosters with anise oil especially at the level of 500 ml / ton of diet. |