BUDDING OF DATE PALM (Phoenix dactylifera L.) C.V HILLAWI In vitro

AL-Khlifa, A.A.S.

	BUDDING OF DATE PALM (Phoenix dactylifera L.) C.V HILLAWI In vitro
IRAQ JOURNAL OF AGRICULTURE
Article 1, Volume 14, Issue 6, 2009, Pages 0-0
Author
A.A.S. AL-Khlifa
Abstract
This study was effectuated at Date Palm tissue culture laboratory (Date Palm Research Center –Basrah University) during 2007, in the aim to formation of lateral buds from quarter apical buds of date palm offshoots c.v Hillawi. The quarter apical buds treated pre culture by immerged in solution of plant growth regulators composed of 5, 10 mg/l (2iP) for 5, 10 minutes. these quarters were cultured on nutrient medium of (MS)salts full strength supplemented 30 g/l sucrose,1 g/l activated charcoal, 6 g/l agar, 1 mg/l NAA and several concentration of cytokinins (2iP or BA) 5,10 mg/l was tested . Cultures were incubated in darkness for two months at 27±1c° and sub cultured was done every four weeks then the culture was transfer to lights at 1000 lux at periods16 hours daily the results showed that: 1- The immerged of the quarter apical buds in 10mg/l (2iP) for 5 and 10 minutes lead to formation of lateral buds in few period was reached 117 and 118 day respectively. 2- It showed the nutrient medium with 5 mg/l (2iP or BA) was stimulated to formation of lateral buds in short period significant compared to the other concentration. 3- The immerged treatment at 10minutes in 10mg/l (2iP) led to have higher number of lateral buds when they cultured on nutrient medium with 5 mg/l (2iP).

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