PROPAGATION OF ROUGH LEMON (Citrus jambhiri lush) IN Vitro

M. Bader, S.; H. Taha, F.; S. Hammad, M.

	PROPAGATION OF ROUGH LEMON (Citrus jambhiri lush) IN Vitro
IRAQ JOURNAL OF AGRICULTURE
Article 1, Volume 14, Issue 6, 2009, Pages 0-0
Authors
S. M. Bader; F. H. Taha; M. S. Hammad
Abstract
This study was conducted in the tissue culture laboratories / Palm and Dates Department / State Company for Horticulture and Forestry / Ministry of Agriculture. It aims to propagate the rough lemon (Citrus jambhiri Lush.) rootstock in vitro. Different explants from mature trees (shoot tips, single nodes and internodes) were used at different times. The medium Murashige and Skoog (MS) was used in all growth stages. Results showed that the summer time (july) was the best in giving a high percentage of response, which was 80% for shoot tips and 77.77% for nodes at the initiation stage. While no response was recorded with internodes which then discarded . For multiplication, the highest mean of shoots (2.1) and length (0.98 cm) was achieved using 1 mg / l for both Benzyl adenine (BA) and Gibberellic acid (GA3). In the rooting stage, the highest mean of rooting percentage 50%, mean of roots (0.8) root / explant and length (0.98) was found on solid medium containing 1/2 MS salts and 1 mg / L Naphthaleneacetic acid ( NAA). All rooted shoots were transplanted in small pots containing sterilized loam and peatmoss (1: 1) after the roots were washed with distilled water and then treated with fungicide. After two weeks the % of plants survived acclimatization was 60%.

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