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Study on some metabolites composition of ovarian follicular fluid in relation to follicular size in local cows | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 6, Issue 3, 2014, Pages 123-130 | ||
| Abstract | ||
| Abstract : The aim of this study was to estimate the some metabolites composition of ovarian follicular fluid from different sized follicles and it's relationship with follicular size in local cows. Ovaries were collected (120 ovary) from 60 adults non-pregnant cows which started slaughtered at abattoirs of province of Babylon during the period from September 2013 to March 2014. The ovaries were transported to the laboratory within 2 hours post slaughter. Follicular fluid was aspirited from small (3-5mm), medium (6-10mm) and large (11-20mm) follicles and stored at -5ºC prior to assay. The follicular fluid samples were analyzed for cholesterol, triglycerides, total protein and glucose, using commercially available kits. The result showed that the mean concentration of cholesterol and glucose in follicular fluid of large follicles were significantly higher (P<0.05) than the follicular fluid in small follicles. While the mean concentration of triglycerides and total protein in ovarian follicular fluid of large follicles were significantly lower (P<0.05) than follicular fluid in small follicles. | ||
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