Effect of Explant type and Benzyladenine on Culture Initiation and Multiplication of Three Strawberry's Cultivars

	Effect of Explant type and Benzyladenine on Culture Initiation and Multiplication of Three Strawberry's Cultivars
Euphrates Journal of Agriculture Science
Article 1, Volume 6, Issue 4, 2014, Pages 1-13
Abstract
Abstract: The study was conducted to examine the effect of explant type and Benzyladenine (BA) on culture initiation and multiplication of three Strawberry's (Fragaria x ananassa Duch.) cultivars using plant tissue culture technique. Several experiments were conducted comprising study of two explant types (apical meristem and runner tip) of the three cultivars: Festival, Robican and Sweet charly response to surface sterilization and in vitro culture under various concentrations of the cytokinine BA. Results indicated that the lowest contamination percentage (5%) was achieved in Festival cv. which was significantly deferred from Robican and Sweet charly cvs. (15%), while apical meristem was lower contaminated (6.6%) than runner tip (16.6%). Murashige and Skooge (1962) (MS) medium with 0.5 mg/L BA gave the highiest percentage of in vitro response of runner tip for Festival cv. (90%) and the highiest number shoots/explant (6.2) while apical meristem for the same cultivar gave 5.1 shoots/explants. In multiplication stage, Festival cv. was the best and gave 12.43 shoots. BA at 1.0 mg/L was the best concentration and gave 16.7 shoots. The highest multiplication mean was achieved for culturing Robican cv. shoots on MS medium supplemented with 1.0 mg/L BA plus 0.1 mg/L IBA (17.6 shoots). Festival cv. record highest number of leaf/plantlet (24.02). The highest average of leaf number was achieved in Robecan (33.9 leaf). The above results is useful in the micropropagation protocol of Strawberry.

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