EFFICIENCY OF SOME BIOLOGICAL FACTORS AND PLANT EXTRACTAS AGAINST THE FUNGUS RHIZOCTONIA SOLANI KUHN REASONED ROOT ROT DISEASE OF SOYBEAN

	EFFICIENCY OF SOME BIOLOGICAL FACTORS AND PLANT EXTRACTAS AGAINST THE FUNGUS RHIZOCTONIA SOLANI KUHN REASONED ROOT ROT DISEASE OF SOYBEAN
Euphrates Journal of Agriculture Science
Article 1, Volume 6, Issue 4, 2014, Pages 183-197
Abstract
Abstract: The Present study aimed to investigate the isolation and diagnosis of the Soybean roots rotting causative agent with the evaluation of some agents of bioprotection and its protection efficiency under the Laboratory and the Lath house environment . Results indicated that isolation from efected Soybean roots the presence of Rhizoctonia solani Fungus which is presented in recurrent condition from all samples that brought Karbala province fields. The fungus isolates referred that the pathogenic isolate of R.solani showed of Soybean seeds cultivation which was significantly surpassed for increasing the pathogen city severity to 100% as compared to the control which was zero%0 ,I n addition the pathogenic fungus led to significant reduction of lengths with the wet and dry weights of the greenish and root parts of the plant . Antagonistic ability of Azotobacter chroococcum bacteria indicated a high efficiency inhibition of the pathogenic fungus by 76.66% . Also, alcoholic and water extract of (bee bread Borago officinalis L plant and the spikyAmaranthus spinosus and normal cock comb) Amaranthus retroflexus had an efficacy in inhibition of the pathogenic fungus on PSA medium Moreover, the bee bread plant extract gave the best efficiency as compared with the other extracts .whenethe inhibition percentage of the pathogenic fungus of the water extract of bee bread plant was (15%) 57.4% , while the alcoholic extract was (10%) 75.6% . Topsin M herbicide gave a high inhibitory action at the concevtrotion (1%) on the PSA medium at the presence of the pathogenic fungus which was 80.37% in comparison with the control . Lath house results indicated that all treatments caused reduction in the infection percentage and the severity of infection .The interaction between the A.chroococcum with the alcoholic extract of bee bread plant with the bacteria and the water extract of the bee bread plant showed an efficiency in reduction of infection rate with its severity in root rotting that cosed by R.solani . The inhibition percentage of the two treatments was 26.7,10.00,46.70and 16.67% respectively in comparison with the pathogenic fungus treatment alone when the infection percentage and severity was reaened 100% .All treatments led to significant elevation of the studded plant growth parameters as compared with the pathogenic fungus alone , the dry and wet weights of the green and root parts in the two treatments of the bacterial interaction with the alcoholic extract of the bee bread plant and the bacterial treatment with the water extract of the bee bread plant as well by the presence of the fungus were 3.63, 1.81. 3.40 and 1.72gm respectively and the dry weight were 2.08 , 0.65, 2.04 , and 0.58gm respectively.

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