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THE IN VIVO STABILITY OF DIRECTLY INJECTED NAKED DNA AND CALCIUM PHOSPHATE CONJUGATED DNA INTO HENS | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 7, Issue 1, 2015, Pages 1-10 | ||
| Author | ||
| Mohammed Baqur S. A. Al-Shuhaib | ||
| Abstract | ||
| ABSTRACT: There is a crucial need for a low cost, non-biohazard horizontal gene transfer method in avian to produce enhanced genetic traits. The delivery of recombinant DNA into hen's tissues has been confirmed for practical use to promote the desired productive features. But still has several limitations such as expense, and the requirement of specialized devices for manufacture. Therefore, a low cost and calcium phosphate tool was devised, and its efficiency was tested by its direct exposure to DNase. Thus, to investigate the stability of the injected DNA through this method, hens were used as biomarkers in this study. Twenty mature hens were randomly chosen. They were directly injected by calcium phosphate – DNA and naked DNA methods. Then, blood samples were withdrawn after one day, three days, and one week of injection. DNA samples were extracted from blood in each case. Afterwards, both methods of direct gene injection were compared using polymerase chain reaction to explore the persistability of injected DNA in both cases. Though the naked DNA has initially shown much more penetration compared with its calcium phosphate counterpart, the later method has shown more stability in vivo. Nevertheless, both in vivo injection methods aren’t practical enough for long term in vivo gene expression. Keywords: comparison, naked DNA, calcium phosphate, direct injection | ||
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