PRODUCTION AND ESTIMATION OF HYOSCYAMINE AND HYOSCINE IN Datura stramonium USING PLANT TISSUE CULTURE TECHNIQUE

S. Shawkat, M.; Bader, S.M.

	PRODUCTION AND ESTIMATION OF HYOSCYAMINE AND HYOSCINE IN Datura stramonium USING PLANT TISSUE CULTURE TECHNIQUE
IRAQ JOURNAL OF AGRICULTURE
Article 1, Volume 14, Issue 6, 2009, Pages 0-0
Authors
M. S. Shawkat; S.M. Bader
Abstract
The quality and quantity of tropane alkaloids were studied in different parts of Datura plant (Datura stramonium) and in callus induced from leaves. When an investigation about the main dominant tropane alkaloids represented in two compounds (Hyoscyamine and hyoscine) in the extracts of leaves, seeds, stem and root, it was appeared that the concentrations of these compounds distributed in the different organs of the plant according to their nature and function. They concentrated in leaves and seeds while their concentration decreased in root and stem. It was possible to induce callus from the leaves cultured in MS medium supplemented with growth regulators represented in two treatment; the first one consisted of K; 2,4-D and NAA (1.1 and 0.5 mg/ liter) and the second consisted of BA and NAA (0.5 and 1 mg/liter), it was observed that the response of D. stramonium in callus inducing reached 85% for the first treatment and 75% for the second one. Also it was observed that the first treatment was superior to the second one in the fresh weight of the growing callus as it reached 5.8 and 4.95 g/ 10 cm3 of the medium respectively. The extracts of the callus induced from leaves of Datura plant were superior in high concentration of hyoscyamine and hyoscine which were 8.101 and 7.090 mg/g dry weight for the first treatment while they were 7.407 and 6.727 mg/g dry weight for the second treatment respectively when compared with their concentration in the leaves of the plant, 0.198 and 0.140 mg/ gm dry weight.

Statistics Article View: 136 PDF Download: 74