Influence of some environmental conditions on Alternaria alternata growth and alternariol AOH production

	Influence of some environmental conditions on Alternaria alternata growth and alternariol AOH production
journal of kerbala university
Article 1, Volume 10, Issue 4, 2014, Pages 84-90 PDF (0 K)
Abstract
This study included the influence of some environmental factors, temperature, pH, type of culture media, light and dark on the growth of A. alternata and production Alternariol AOH. The results showed that temperature had a significant effect (p<0.05) on the A. alternata growth and the optimum temperature was 25 ºC (8.5 cm for colony diameter) and the maximum temperature was 35ºC, minimum temperature was 20ºC. The results showed that ,temperature had a significant effect (p<0.05) on the on AOH production and the optimum temperature was 30 ºC (141.5 microgram/ml). pH effected growth where the best growth of A. alternate was at pH 6 giving 8.5 cm for colony diameter and a difference was significant at the p< 0.05 for other pH when the pH was 8 less than the growth rate 4.3 cm for colony diameter as well as pH affected production of AOH by significant differences at pH was the highest production 116.1 micrograms/ml and less production was at pH 8 giving 57.8 microgram/ml. This results also showed that, there are significant differences in the rate of growth of A. alternata depending on the type of culture media, PSA showed significant differences among others of growth 8.5 cm for colony diameter and decreased rate growth in other types of media lower growth rate of 4.0 cm for colony diameter for Sabourd agar. PAS gave best amount of production of toxin 112.8 microgram/ml. Dark condition showed a significant increase in growth of A. alternata 8.2 cm for colony diameter while 6.5 cm under light conditions, So a significant increase for AOH production was in dark 182.8 microgram/ml, while in light was 74.5 microgram/ml.

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