ISOLATION OF PSEUDOMONAS AUREAFACIENS AND EVALUATION ITS INHIBITORY EFFICIENCY AGAINST SOME FUNGI IN VITRO AND ITS ROLE IN IMPROVING ZEA MAYS GROWTH

A. A. Ratha, H.; .T. Hamed, A.

	ISOLATION OF PSEUDOMONAS AUREAFACIENS AND EVALUATION ITS INHIBITORY EFFICIENCY AGAINST SOME FUNGI IN VITRO AND ITS ROLE IN IMPROVING ZEA MAYS GROWTH
IRAQ JOURNAL OF AGRICULTURE
Article 1, Volume 14, Issue 9, 2009, Pages 0-0
Authors
H. A. A. Ratha; A. .T. Hamed
Abstract
This study was conducted to isolate and identity pseudomonas aureafaciens from different of sites soil in AL- Anbar governorate and evaluate its inhibitory efficiency against the growth of the pathogenic fungi Rhizoctonia solani, Alternaria sp., pythium sp. And its role in improving the growth of zea mays. Bacteria were isolated from soil samples by using serial dilution and plating on KB and PDA, diagnostic study depended on the morphological, cultural and biochemical tests. Results showed that 25 isolates were belonging in its characteristic to p.aureafaciens with the superiority effect of isolates p14 in its antifungal effect. The diameter of R. solani, Alternaria sp., pythium sp. Colonies were (4,4,4.5) mm (5.5,4,4.4) mm and (6,5.6,5.6) mm respectively with the three bacterial isolates No. 14 , 21 and 24 in compare with the diameter of these three fungi in control which reach to (89,88 and 67)mm. Results showed that treated of corn seeds with the two isolates of p.aureafaciens p14 and p21 and planning it's in non sterily soil caused to superiorty effect of p14 isolate in increasing the germination percentage, length of plant, chlorophyll content, leaf area, phosphorus content, Nitrogen content compared with the control treatment. The germination percentage age was 95%, 65% for the p14 and control respectively, while phosphorus content was 1.5 and 0.18 mg/100g plant for the p14 and control respectively and Nitrogen content was 5 and 2.5 mg/100g plant for the same treatments above.

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