- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Cytotoxic Effect of Gliotoxin, Hemolysin, Protease and Melanin purified from Aspergillus fumigates on REF Cell Line, in vitro Study | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 9, Issue 2, 2015, Pages 4-10 | ||
| Authors | ||
| Batool Omran Theeb; Farooq Ibrahem Mohammad; Abdulkareem Jasim Hashim | ||
| Abstract | ||
| Aspergillus fumigates especially clinical isolate produces a series of toxic substances and proteinaceous hemolysin, protease and pigment like melanin which appear to act in an additive and synergic way on cells. In this study, gliotoxin, hemolysin, Protease, and Melanin were used in an experimental model to study their Cytotoxic activity by evaluating their effect on REF cell line (Rat embryonic fibroblast ), for exposure time of 24 hrs at three different concentrations of each compounds triplicate of each concentration were used, cytotoxicity of the purified compounds were active against REF cell line under study and a toxic effect was clear with a significant difference at the level of probability (p≤ 0.05) and this effect was contrasted among different concentrations for each purified compound growth inhibition of REF cell line was increase gradually with the increase of compound concentration. | ||
| Keywords | ||
| Gliotoxin; Hemolysin; Protease; Melanin; Cytotoxic activity | ||
|
Statistics Article View: 170 PDF Download: 53 |
||