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PCR Detection of Some ESBLs (bla) Genes in Pseudomonas aeruginosa Isolated from Burn’s Units in Bagdad Hospitals | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 9, Issue 2, 2015, Pages 74-80 | ||
| Authors | ||
| Asmaa A. AL-Kaisse; Amina N. AL-Thwani; Rabab Q. AL-Segar | ||
| Abstract | ||
| Multi drug resistant (MDR) and Extended Spectrum Beta Lactamase (ESBLs) of Pseudomonas aeruginosa were detected. Pseudomonas aeruginosa is a bacterium responsible for severe infections in burn’s units, plasmid DNA analysis and encoded many types of genes responsible for beta-lactamases. To determine the type of genes responsible for beta-lactam broad spectrum in P. aeruginosa strains isolated from 100 swabs of burn’s units environment, using a molecular methods (PCR) by primers specific to ESBLs (bla ) genes oxacillin hydrolyzing capabilities OXA-10, OXA-4 and Vietnam Extended-Spectrum β-Lactame VEB-1. The results revealed that 15 strains were isolated from burn units environment. All of 15 (100%) were positive OXA-10 and only one (6.6%) for OXA-4 while the other gene VEB-1 was found in 6 (40%) isolates. | ||
| Keywords | ||
| Burn; pseudomonas aeruginosa; PCR; Resistance genes | ||
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