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Inactivation of Staphylococcal Virulence Factors Using a Diode Laser light-Activated Antimicrobial Agent | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 9, Issue 2, 2015, Pages 94-101 | ||
| Authors | ||
| Norrya A. Ali; Dheyaa Khaleel Ismael; Rafeef Yousif Rasheed | ||
| Abstract | ||
| Total of 120 samples were collected from different body sites and lesions for patients from both sexes. There were 78 isolates (65%) Staphylococcus aureus from them 37 isolates were Methicillin-resistant Staphylococcus aureus (MRSA). Each isolate was exposed to diode laser 632nm wavelength with 300µM of methylene blue (MB) at 458.6 watt/cm2 for 15 minutes, both phenotypic and genotypic changes in virulence factors and the antibiotic-resistance were evaluated before and after irradiation with laser light. It was observed that the effect of sub-lethal dose on the antibiotic sensitivity was isolate-dependent. In general, results showed large variations in the susceptibility of antibiotic. Exposure to diode laser increased resistance to Vancomycin, in contrast increased sensitivity to Methicillin, Ciprofloxacin, and Norfloxacin. Also Results showed decreases the activity of β-haemolysin, with 33 (90.3%) isolates of S. aureus in comparison to the control as shown in blood agar method and haemolytic titration assay, but there was no effect on thermonuclease enzyme after irradiation. Detection of three genes represented in MRSA isolates by a confirmatory test was carried out using PCR technique. The results of the PCR amplification of mecA gene noted that it was present in 27(72.2%) S.aureus isolates and 14(83.3%) isolates showed reduction in β-toxin production after exposure to laser light, with no altered or deficiency in thermonuclease gene (nuc). | ||
| Keywords | ||
| irradiation; antibiotic susceptibility; beta; haemolysin; Methylene blue; diode laser | ||
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