Relationship between Some Bacterial Adhesion Ability to  Females' Epithelial Cells and Diabetes

Sh. AL-Salihi, Siham

	Relationship between Some Bacterial Adhesion Ability to Females' Epithelial Cells and Diabetes
Al-Mustansiriyah Journal of Science
Article 1, Volume 26, Issue 1, 2015, Pages 8-14
Author
Siham Sh. AL-Salihi
Abstract
The aim of this research is to determine the relationship between adhesion ability of important bacteria causes urinary tract infection to females' epithelial cells in diabetic and non diabetic women. For this study 135 female were eligible, their ages were 10-60 years old. They were divided into six groups; two of the groups were consist of 15 healthy married and 15 healthy single women whom were used as controls. The other four groups included married and single diabetic women (15 females for each group), married and single diabetic women with other type of diseases (50 and 25 female for each group respectively). The adhesion property of the bacteria (E. coli, Klebsiela pneumoniae, Proteus mirabilis, and Staphylococcus aureus) was tested on the females' uroepithelial and buccal cells. Regarding the age group, the significantly highest adhesion rate to uroepithelial cells were in 50-60 years and 40-49 years age groups for all the bacteria, while to buccal cells the highest were in 10-19 years and 50-60 years groups. The bacteria had adhered more significantly to uroepithelial and buccal cells from diabetic than none diabetic women, and even more in married diabetic women than in married none diabetic one. Adding mannose or sucrose to the incubation media had significantly decreased the adhesion ability of E. coli, K. pneumonia, and P. mirabilis but not affected the S. aureus. The mannose was significantly decreased the adhesion ability of the bacteria than sucrose. The adhesion ability of the bacteria was increased to uroepithelial cell in diabetic married and single women with UTIs and kidney problems, while for buccal cells it was increased in influenza suffering patients. Increasing incubation time had increased the adhesion rate of the bacteria, the highest was for 60-75 min. and the lowest was for 15 min. for each of uroepithelial and buccal cells. We can conclude that adhesion ability of bacteria was increased to uroepithelial cell in diabetic married and single women.

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