Genetic Comparative Study of Staphyolococcus aureus& Staphylococcus epidermidis Isolated from Wounds, Burns and Skin Flora

S. Abdul-Kareem, Hala; S. Husain, Ali

	Genetic Comparative Study of Staphyolococcus aureus& Staphylococcus epidermidis Isolated from Wounds, Burns and Skin Flora
Iraqi Journal of Science
Article 1, Volume 56, Issue 1, 2015, Pages 708-712
Authors
Hala S. Abdul-Kareem; Ali S. Husain
Abstract
One hundred specimens from wounds, burns, and skin swabs were collected from patients laying and attended to Balad general hospital. It was found that 50 isolates belong to Staphylococcus spp., 38 isolates were identified as S. aureus and 12 isolates were identified as S. epidermidis according to microscopic, cultural and biochemical testing. The study of seven extracellular enzyme as virulence factors including the enzymes: urease, lipase, DNase, haemolysin, coagulase, β-lactamase, and lecithinase. Reavealed that 100% of S.aureus had the ability to produce these enzymes, while S. epidermidis isolates were unable to produce the enzymes DNase, lipase, coagulase, but they were capable to produce haemolysin, urease, lecithinase, and β-lactamase, the range for production of these factors were 58.33 %,80,%, 10,%, and 45% respectively. Eighteen Staphyolococcus isolates were selected according of their ability for production most of studies virulence enzymes for detection of genes encoding for the enzymes heamolysine (hly) and coagulase (coa) by using of polymerase chain reaction (PCR) technique. The Gene hIy was detected in all isolates and coa was detected in 14 isolates only
Keywords
Staphylococcus aureus; PCR; virulence factor

Statistics Article View: 164 PDF Download: 122