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The using of antisperm antibody assay as a predictive diagnostic test of male infertility | ||
| Magazine of Al-Kufa University for Biology | ||
| Article 1, Volume 7, Issue 1, 2015, Pages 131-141 | ||
| Authors | ||
| Ali A. Al-Fahham; Yahya K. M. Al-Sultani; Abdulzahra K. Muhammad-Ali | ||
| Abstract | ||
| Assessment of male infertility is the first step in resolving this worldwide problem . The aim of the present study is to investigate the predictive value of using ELISA (Enzyme Linked Immuno-Sorbent Assay) test for serum and seminal plasma Antisperm antibodies (ASA) as a complementary tool to assist in diagnosis of infertility . This study was carried out between January 2013 and November 2013 including one hundred (100) selected infertile men who attended to fertility Center in Al-Sadr Medical City . The study also included twenty (20) healthy volunteer fertile as a control . The results showed that the incidence of serum and seminal ASA in infertile men is significantly (p<0.05) higher than that in control fertile men. the incidence of serum and seminal ASA is significantly (p<0.05) higher in normozoospermic patients than that in control fertile men . There was a high significant negative correlation (p<0.01) between the concentration of serum and seminal ASA in infertile men and each of : sperm motility, progressive motility, while a high significant positive correlation (p<0.01) is seen between serum and seminal ASA in infertile men and sperm agglutination, and a significant positive correlation (p<0.05) with seminal WBC count . The study concluded that ELISA ASA assay can be routinely used as a complementary test to diagnose infertility . | ||
| Keywords | ||
| male infertility; ELISA; seminal plasma; Antisperm antibodies | ||
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