Extraction of Aloin from Aloe Vera plant and study its effect in Micronucleus Formation in Acute Lymphoid Leukemia

S. Alumairi, Sumaya; S. Shawkat, Muayad; A. Alaubydi, Mouruj

	Extraction of Aloin from Aloe Vera plant and study its effect in Micronucleus Formation in Acute Lymphoid Leukemia
Iraqi Journal of Science
Article 1, Volume 56, Issue 1, 2015, Pages 731-737
Authors
Sumaya S. Alumairi; Muayad S. Shawkat; Mouruj A. Alaubydi
Abstract
Aloin extracted into alcohol-rich phase with high extraction efficiency, meanwhile majority polysaccharides, proteins, mineral substances and other impurities were extracted into salt-rich phase. Partitioning of AQs[Anthraquinones] is dependent on hydrophobic interaction, hydrogen bond interaction, and salting-out effect in Aqueous tow –phase system [ATPS]. Aloin was partially purified by using 1-propanol [NH4] 2SO4, the use of this solvent showed high efficiency 90.61% compared with other solvent [2-propanol and ethanol]. The concentration of aloin detected by HPLC technique, which reached to 91.84% as focus turns out that there is compatibility between the sample and the standard in shape and retention time which reached [1.462] compared with the standard [1.465]. After the treatment of blood leukemic patient cultures, three concentrations of aloin were used [100, 200 and 400] µg/ml, the last concentration showed the ability to reduce the coefficient of micronucleus formation to [0.0062] micronucleus/cell, compared with non- treated culture which reached to [0.0236] micronucleus/cell.

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