- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Detection of Codon 12/13 g.6262G>A Mutation of H-ras Gene in Iraqi Bladder Carcinoma Patients | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 14, Issue 1, 2015, Pages 44-52 | ||
| Authors | ||
| Amer M. Kraidi; and Ahmed A. Suleiman; Abdul Hussein M. AL-Faisal | ||
| Abstract | ||
| Abstract: DNA was extracted from blood and urine samples from 45 patients with bladder carcinoma (age 20-87 years) in addition to samples from 25 apparently healthy persons as controls. Restriction fragment length polymorphism (RFLP) analysis was performed to determine genotypes of the H-ras codons12,13 using Msp1 enzyme. The healthy results showed that two fragments (165 bp and 55 bp) were produce from the digestion with the enzyme for H-ras codon 12/13. These results indicated that the PCR amplified region of the codon 12/13 has one restriction site for the enzyme Msp1. The molecular analysis of the patient samples revealed that among 45 patients included in this study, 28 patients (62.2%) were with normal pattern (165 bp and 55 bp) and 17 patients (37.8%) were homozygous mutants (g.6262G>A). The frequency of g.6262 C>G mutation in patients was significantly higher than in apparently healthy subjects (37.3% versus 0%, OR= 0.033; X2=0.966*, P<0.01). | ||
| Keywords | ||
| Bladder carcinoma; RAS; MSP; RFLP | ||
|
Statistics Article View: 180 PDF Download: 53 |
||