Rooting response of good mungbean cuttings for 72 h in D/H2O was declined by (65.3%) and (41.3%) in terms of root number and root length respectively compared to Complete Hoagland Solution (C.H.S) . Howeever , the absence of any Macro- and Micro – elements has significantly a negative impact on root length that developed in aged cuttings at probability level (0.01) except absence of calcium (Ca) .
In addition , results were showed that rooting response was also declined in cuttings supplied with all Hoagland solution lacking for (HSLF) Macro – and micro elements at (0.05) level. The lower values , in particular , with cuttings aged in HSLF (Zn) and (K) caused reduction by 58.4% and 49.8% respectively compared to the control (C.H.S) , except the significant increase (32.4%) in the solely treatment of HSLF (B) that stopped partially the proceses that occurred during ageing period and causes a decline in rooting response. The later case was conicided with an increase of free IAA to its max. level (1.016) mM , moderate increase in free ABA , in addition to a decrease of free cytokinin to the lowest level in primary leaves.
Moreover , in terms of anti – oxidants , total ASA was increase in HSLF (B) in addition to decrease of catalase and SOD activity while IAA-O was increased moderetly in a rate that coincided with increase in IAA level as mentioned above. In addition to unchanged chlorophyll content , decline of transipiration rate to a lowest level that accompanied a decline in protein content (43.1%) and total carbohydrate (20.3%) by maintaining high ratio of N/C in primary leaves of aged cuttings.
Key words : Adventitious rooting , Ageing , Ascorbate , Auxins , Carbohydrate , CAT , Macro – nutrients , Micro-nutrients , Modefied Hoagland Solution , Plant hormones , Mungbean cutting , Proline , Protein , Transpiration . |