Estimating the inhibitory effect of Lactobacillus isolated from vagina against some pathogens of genital infections in group of women.

.S. Hindal, Azhar; .A. Ali, Sameer

	Estimating the inhibitory effect of Lactobacillus isolated from vagina against some pathogens of genital infections in group of women.
Iraqi Journal of Science
Article 1, Volume 56, Issue 2, 2015, Pages 1588-1593
Authors
Azhar .S. Hindal; Sameer .A. Ali
Abstract
Thirty- five vaginal swab samples were obtained from women vagina.All samples were subjected to conventional morphological and cultural characteristics, isolates distributed between Lactobacillus acidophillus(23)and Lactobacillus fermentum (12). Antibacterial activities were done by well diffusion and blank disk method. The Lactobacillus was used as aprobiotic treatment against bacteriaisolates from vagina .The isolated bacteria had strong activity against indicator strains.The results showed that Lactobacillus which was isolated from vagina by well diffusion method was effective against pathogenic isolates more than the Lactobacillus isolated by blank disc method , the highs inhibitory effect of Lactobacillus isolates by well give an inhibition zone reached to(18)mm , while the Lactobacillus by disc was lower with inhibition zone (12-15) mm. Also the results revealed that Lactobacillus spp. whole cell of Lactobacillus was more effective against pathogenic isolates more than usedsupernatant . The supernatant did not show any activity when was treated with NaOH and adjusted to pH 7. This indicates that the organic acid produced by the Lactobacillus isolates was may be actually responsible for the inhibition of the indicator bacteria.The result show that theLactobacillus strains could be considered asapotential antimicrobial probiotic strains against some human vaginal pathogens and should be further studied for their human health benefits.
Keywords
Lactobacillus; Probiotic; Vaginal flora

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