L. Arif, G., O. Alfalahi, A. (2014). Using DNA Sequencing to Detect and Verify Genetically Modified Maize (Zea mays L.) in Iraq.. , 7(3), 1-7. doi: 10.37652/juaps.2013.104793
Gaith L. Arif; Ayoub O. Alfalahi. "Using DNA Sequencing to Detect and Verify Genetically Modified Maize (Zea mays L.) in Iraq.". , 7, 3, 2014, 1-7. doi: 10.37652/juaps.2013.104793
L. Arif, G., O. Alfalahi, A. (2014). 'Using DNA Sequencing to Detect and Verify Genetically Modified Maize (Zea mays L.) in Iraq.', , 7(3), pp. 1-7. doi: 10.37652/juaps.2013.104793
L. Arif, G., O. Alfalahi, A. Using DNA Sequencing to Detect and Verify Genetically Modified Maize (Zea mays L.) in Iraq.. , 2014; 7(3): 1-7. doi: 10.37652/juaps.2013.104793

Using DNA Sequencing to Detect and Verify Genetically Modified Maize (Zea mays L.) in Iraq.

Journal of University of Anbar for Pure Science
Article 1, Volume 7, Issue 3, 2013, Pages 1-7    PDF (535.89 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2013.104793
Authors
Gaith L. Arif1; Ayoub O. Alfalahi* 2
1Al-Nahrain University -Forensic DNA Training Unit
2University of Anbar - College of Agriculture
Abstract
Abstract: Polymerase chain reaction (PCR) was used to detect genetically modified maize. The results revealed that 10 out of 72 maize DNA samples were genetically modified. Verification of PCR ampliconse indicated that all GM maize were of MON810 type engineered with cryIAb gene under the control of the cauliflower mosaic virus P35S promoter and NOS terminator. Direct DNA sequencing for three out of the ten detected GM maize confirm the presence of P35S promoter and NOS terminator. Also, the constructed alignment of both genetic elements in comparison with their respective sequence in the NCBI database, indicated complete identity level for P35S, whereas nucleotide mismatches was detected in two sites of NOS118 terminator both on the forward strand. Meanwhile one site was corrected by the reverse strand, the other site may be resulted from Tag polymerase mismatches. These results approved the highly conservativeness of both detected elements and the completely absence of single nucleotide mutations. Results revealed clearly that nonauthorized GM maize was entered the national agriculture sector without authorities permission, which maximize risks of spreading GM maize all around the country and need to act immediately by setting strict legislation and monitoring system.
Keywords
GM maize; DNA sequencing; NOS terminator and P
References
  1. Daniell, H. 2007. Transgene containment by maternal inheritance: Effective or elusive? PNAS, 104 (17) : 6879–6880.
  2. Matsuoka, T., H. Kuribara, K. Takubo, H. Akiyama, H. Muira, Y. Goda, Y. Kusakabe, K. Isshiki, M. Toyoda and A. Hino. 2002. Detection recombinant DNA segments introduced to genetically modified maize (Zea mays). J. of Agric. and Food Chem., 50: 2100-2109.
  3. Sahoo, L., J.J. Schmidt, J.F. Pedersen, D.J. Lee and J.L. Lindquist. 2010. Growth and fitness components of wild x cultivated Sorghum bicolor (Poaceae) hybrids in Nebraska. Amer. J. of Bot., 97(10):1610-1617.
  4. Alfalahi, A.O. and F.W. Abdulqahar. 2010. Biota gene flow and genome contamination. The Iraqi J. Agric. Sci. 41(2):126-149.
  5. Rimachi, L.F., J.A. Delgado, Y.A. Villasante and R. Ortiz. 2011. Detecting adventitious transgenic events in a maize center of diversity. Electronic J. of Biotech., 14(4):1-12.
  6. Huang, H-Y. and T-M. Pan. 2004. Detection of genetically modified maize MON810 and NK603 by multiplex and Real-time polymerase chain reaction methods. J. Agric. Food Chem., 52: 3264-3268.
  7. Ahmed, F. 2002. Detection of genetically modified organisms in foods. Trend in Biotech., 20(5): 215-223.
  8. Holst-Jensen, A., S.B. Ronning, A. Lovseth and K.G. Berdal. 2003. PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal. Bioanal. Chem., 375: 985-993.
  9. Gurel, F., E. Arican, N. Gozukirmizi and S. Ari. 2011. Recent molecular tools for detecting transgenic events in genetically modified (GM) crop products. Sci. Res. and Essays, 6(24): 5091-5099.
  10. Shrestha, H.K., K-K. Hwu and M-C. Chang. 2010. Detection of genetically modified maize (Zea mays L.) in seed samples from Nepal. Afric. J. of Biotech., 9(34): 5581-5589.
  11. Hernandez, M., D. Rodriguez-Lazaro, D. Zhang, T. Esteve, M. Pla and S. Prat. 2005. Interlaboratory transfer of a PCR multiplex method for simultaneous detection of four genetically modified maize lines: Bt11, MON810, T25 and GA21. J. Agric. Food Chem., 53(9):3333-3337.
  12. Shrestha, H.K., K.K. Hwu, S.J. Wang, L.F. Liu and M.C. Chang. 2008. Simultaneous detection of eight genetically modified maize lines using a combination of event- and construct-specific multiplex-PCR technique. J. Agric. Food Chem., 56(19): 8962-8968
  13. Nguyen, C.T., R. Son, A.R. Raha and O.M. Lai. 2008. Detection of genetically modified organisms (GMOs) using molecular techniques in food and feed samples from Malaysia and Vietnam. Int. Food Res. J., 15(2): 155-166.
  14. Lipp, M., A. Bluth, F. Eyquem, L. Kruse, H. Schimmel, G. Eede and E.  Anklam. 2001. Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs. European Food Res. and Tech., 212: 497-504.
  15. Rosati, A., P. Bogani, , A. Santarlasci and M. Buiatti. 2008. Characterisation of 3´ transgene insertion site and derived mRNAs in MON810 YieldGard maize. Plant Molecular Biology DOI 10.1007/s11103-008-9315-7.
Statistics
Article View: 3,016
PDF Download: 712


Journal Management System. Powered by iJournalPro.com