ROLE OF  CITRULLUS COLOCYNTHIS  FRUITS EXTRACT AND POLY ETHYLENE GLYCOL IN THE INDUCTION  OF   GENETIC VARIATIONS AND DROUGHT TOLERANCE OF SUGARCANE IN VITRO

J. S. Baday, S.; A. H. Al-shemary, I.

	ROLE OF CITRULLUS COLOCYNTHIS FRUITS EXTRACT AND POLY ETHYLENE GLYCOL IN THE INDUCTION OF GENETIC VARIATIONS AND DROUGHT TOLERANCE OF SUGARCANE IN VITRO
The Iraqi Journal of Agricultural Science
Article 1, Volume 46, Issue 4, 2015, Pages 529-538
Authors
S. J. S. Baday; I. A. H. Al-shemary
Abstract
An experiment was conducted plant tissue culture Labs at the College of Agriculture University of Baghdad, during 2013 – 2014 to stimulate in vitro genetic variations and drought tolerance in two cultivars of Sugarcane (VN413,ROC1).The variables were the extract of Citrullus colocynthis fruits at concentrations: 0,50,100,150,200 ml.L-1 and Poly Ethylene Glycol at concentrations :0.0,7.5,15.0, 22.5,30.0 g.L-1. Measurements has taken on average of the number of the vegetative branches before and after use the extract of Citrullus colocynthis fruits treatment and PCR-RAPD test was done results revealed significant differences in average of the number of the vegetative branches after use with the extract of Citrullus colocynthis fruits at 150 ml.L-1 and 30.0 g.L-1 of PEG, is gave before extract treatment 2.9 branch.plant-1,and after treatment 8.2 branch.plant-1. PCR-RAPD test showed differences in banding Pattern and molecular weights in the extract of Citrullus colocynthis fruits treatment and control treatment,in light of the results can be concluded that the extract of Citrullus colocynthis fruits may be have the ability to make a genetic variations and increase bearing shoots to drought stress caused by treatment with PEG in sugar cane, and recommends isolating the substance responsible for causing these variations and diagnosis.
Keywords
plant tissue culture; Extract of; PEG; PCR; RAPD

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