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MOLECULAR AND MORPHOLOGIGAL CHARACTERIZATION FOR SOME CARNATIONSPECIES(Dianthusspp.). | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 46, Issue 5, 2015, Pages 793-801 | ||
| Author | ||
| Janan K. Hussein | ||
| Abstract | ||
| The study includedtwo phases, the first one was carried out at one of Baghdad nurseries on 15/9/2013 sowing the seeds of 12 species ofDianthusspp.( D.caryophyllus, D. chinensis, D. gratianopolitanus, D. barbatus, D. balbisii, D. repens, D. alpinus, D. monspessulanus, D. pavonius, D. deltoids, D. japonicas and D. seguieri)to study some of the qualities phenotypic, and the second phase was carried out atbiotechnology and tissue culture laboratories /College of Agriculture/University ofRazi/Kermanshah/Iran . TheAmplified Fragment Length Polymorphism(AFLP) based on Polymerase Chain Reaction (PCR) with seven primers was implemented. The results of the phenotypic characteristics of the species studied, and there are considerable differences between them where the variation coefficient (C.V.)was very high in all the traits and reached the top rated 85.09 for a number of branches /plant , 57.66 for the number of flowers / plant, 45.08 flower diameter, 32.51 for plant height and 28.66 dry weight of vegetative part . DNA analysis showed that the primers gave a 397 bands including 324 polymorphic bandsand 81.59% polymorphic bands, this confirms the existence of Genetic Distance between the species of Dianthus studied . High Genetic Distance 0.28% D. barbatu and D. caryophyllusand lowest Genetic Distance0.08% betweenD. chinensis and D. japonicas. | ||
| Keywords | ||
| Dianthusspp; AFLP; Molecular characterization; Genetic distance | ||
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