DETECTION  ON DIACETOXYSCIRPENOL  MYCOTOXIN  IN IRAQ ON CORN SEED BY THIN LAYER CHROMOTOGRAPHY (TLC)

. A .Hameed, O; Z. Hussein, H.

	DETECTION ON DIACETOXYSCIRPENOL MYCOTOXIN IN IRAQ ON CORN SEED BY THIN LAYER CHROMOTOGRAPHY (TLC)
The Iraqi Journal of Agricultural Science
Article 1, Volume 46, Issue 5, 2015, Pages 841-846
Authors
O . A .Hameed; H. Z. Hussein
Abstract
This study was conducted to detect Diacetoxyscirpenol (DAS) mycotoxin produced by Fusariumspp in corn seeds and to detect some fungal isolates that produce it.The detection of mycotoxin was archived by Thin Layer Chromotography. Corn seeds collected from some area and tow isolates of F.graminearum and F.culmorum were got from ministry of science and technology to use them of DAS production . A part of corn sampeles was used directly to extract and detect DAS toxin by TLCwhile the other part were used for isolation of Fusarium spp.Results showed that10 isolates from 15 of Fusariumspp produced DAS toxin , some of species wasF.graminearum and F.culmorum also the result of directed detection of DAS mycotoxin showed that DAS associated with most samples and 90.4% of corn seed samples containedDAS toxin for the first time in Iraq , The quantitative detection on DAS mycotoxin by gas liquid chromatography showed deference of Fusarium spp. isolates in DAS production according to site of isolate where is the college of agriculture isolate was the more active in DAS production with 653.89 ng/g , the second isolate in DAS production was Al-Hilla isolate that produced 388.36ng/g from DAS toxin while AL-Rhadwania isolate was 206.34 ng/g and then AL-Basrah isolate that belong to F.graminearum with 65.23 ng/g.This study is the first of recording of those species to secreting DAS toxin in Iraq .We concluded that DAS founded in a high amounts on corn seed in Iraq .We recommend use of suitable storing environment to prevent growth of fungi that producing toxins.
Keywords
Diacetoxyscirpenol; Corn; TLC

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