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Extraction, Partial Purification and Characterization of Inulinase Produced from Aspergillus niger AN20 by Solid State Fermentation | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 14, Issue 2, 2015, Pages 111-122 | ||
| Authors | ||
| Ghazi M. Aziz; Ali J. R. AL-Sa; Zainab W. Abdulameer; | ||
| Abstract | ||
| Inulinase produced by Aspergillus niger AN20 was partially purified using two chromatographic techniques, ion exchange chromatography by DEAE-cellulose and gel filtration by Sephadex G-150. The two steps gave specific activity of 810 U/mg proteins, the purification fold was 8.1 and enzymatic yield was 33.5 %. Some biochemical characteristics of the partially purified inulinase were determined and the results revealed that the enzyme have a molecular weight of 42 KDa., optimum pH for inulinase activity was 4.5, and the pH of the enzyme stability was the range 4.0-8.0. The maximum activity of purified inulinase activity from A. niger AN20 was determined as 50 ᴼC, while the thermal stability ranges from 20-50 Cᵒ. The aim of this study was the extraction and purification of inulinase produced from Aspergillus niger AN20 by solid state fermentation. | ||
| Keywords | ||
| Aspergillus niger; Inulinase; Purification; Characterization | ||
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