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A Molecular Investigation of Phytoplasma (Candidatus Phytoplasma) Infecting Arabic Jasmine (Jasminum sambac) in Iraq | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 56, Issue 4, 2015, Pages 2782-2787 | ||
| Authors | ||
| D. S. Jameel; T.A. Kareem; N. A. S. Al-Kuwaiti | ||
| Abstract | ||
| This study was conducted to investigate phytoplasma causing a virescence disease on Arabic jasmine Jasminum sambac based on microscopy and molecular approaches. Samples were collected from symptomatic Arabic jasmine plants grown in nurseries in Baghdad-Iraq. Specimens from infected plants were prepared and Dienes stained for light microscopy examination. Phytoplasma were detected in infected plants by polymerase chain reaction (PCR) using P1/P7 and SecAfor1/SecArev3 Candidatus Phytoplasma specific primer sets. Light microscopy test showed symptomatic Arabic jasmine plants were phytoplasms infected when phloem tissues were stained with a dark blue color. PCR test confirmed the symptomatic plants were phytoplasms infected when SecAfor1/SecArev3 primers could detect phytoplasms in tested samples. This primer set amplified the 830 bp DNA fragments of secA gene within phytoplasma genome from infected Arabic jasmine samples. Whereas, P1/P7 primer sets could not detect phytoplasms in tested samples. This study is the first molecular confirmation of the phytoplasma disease on Arabic jasmine in Baghdad-Iraq. | ||
| Keywords | ||
| Witches broom; CTAB; Arabic jasmine; Genome | ||
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