DETERMINATION OF CHOLESTEROL IN MILK BY ENZYMATIC METHOD EMPLOYED IN DETERMINATION OF BLOOD CHOLESTEROL

I. Mohamed, Kaseem.

	DETERMINATION OF CHOLESTEROL IN MILK BY ENZYMATIC METHOD EMPLOYED IN DETERMINATION OF BLOOD CHOLESTEROL
The Iraqi Journal of Agricultural Science
Article 1, Volume 41, Issue 3, 2010, Pages 99-104
Author
Kaseem. I. Mohamed
Abstract
The enzymatic method for determination of cholesterol in blood serum was modified to be suitable for determination of cholesterol in milk. Milk samples (0.2 g) are saponified directly (with out extraction of fat) in capped tubes with ( 0.5) Mmethanolic KOH solution by heating for 15 min at80C. After cooling with tap water ,1ml of distilled water was added to the mixtures shaked well for 10 seconds, and the unsaponifiable fractions were extracted with 5ml hexane.The reaction was carried out by using 0.25ml of hexane layer after evaporating the hexane at 37C,mixed for 10 minute with 1ml of enzymatic kit supplied by Spinreact co. [ cholesterol esterase(CE), cholesterol peroxidase, cholesterol oxidase(COD)] ، the concentration determined by using spectrophotometer at 550nm and compare with standard solution at concentration 200mg/100ml. The arithmetic mean of cholesterol content for skim milk, half cream milk, and the whole milk were found(1.29± 0.01,5.30±0.03and12.19±0.04) mgm/100 ml respectively. The precision was 99.89%. The method has been successfully applied to quantitate cholesterol in a variety of milk .

Statistics Article View: 128 PDF Download: 79