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Isolation and Purification of Lactoferrin From Colostrumand From Sweet and Acidic Whey Produce From Cows | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 15, Issue 4, 2015, Pages 185-198 | ||
| Authors | ||
| Mawj Hamid Qaduory; Ethar Zeki Naji | ||
| Abstract | ||
| This study involve separation of lactoferrin from bovine colostrum usingDEAE-Celluloseion exchanger.The optimum pH for the isolation is 8.5 ,standard lactoferrin has given three peaks in the recovery stage and did not show any peak in the washing stage, the first three days of lactation was Characterized that contain highest proportion of lactoferrin, where 93.789% and 88.073% and 60.564% for the first day and the second and third, respectively, compared with the fourth day, fifth, sixth and seventh, which was theratio 37.019% and 30.392% and 30.436% and 47.380%, respectively.lactoferrin was isolated and purified from sweet and sour whey using ion exchanger DEAE-celelluose, the proportion of protein in whey sweet 91.866% and 77.859% in the whey acid,and when using Sephadex G-100 gel the standard lactoferrinandsweet and acid whey with its tow peaks was given one peak,.The molecular weight was determine where was 79.79KDa for standard lactoferrin and lactoferrin purified from sweet and acid whey. | ||
| Keywords | ||
| Lactoferrin; Isolation; Purification; Bovine colostrum; sweet whey; acid whey | ||
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