Incidence and Diversity of Antimicrobial Resistance Profiles of Enterobacteriacae Bacteria in the Cervico-Vaginal Epithelium of Women in Holy Kerbala Province

Mohsin Ali, Mousa; Mohsin Ahmed, Mohanad

	Incidence and Diversity of Antimicrobial Resistance Profiles of Enterobacteriacae Bacteria in the Cervico-Vaginal Epithelium of Women in Holy Kerbala Province
Kerbala Journal of Medicine
Article 1, Volume 8, Issue 2, 2015, Pages 2258-2264 PDF (0 K)
Authors
Mousa Mohsin Ali; Mohanad Mohsin Ahmed
Abstract
background: Although vaginal colonization by bacterial species of theenterobcacteriacae family, the significance of their antibiograms and potential role intransmitting of drug resistance is neglected. Aim of the study: to determine the frequency of occurrence of Enterobacteriacae species and study their antimicrobial susceptibility pattern in cervico-vaginal epithelium of women from kerbala. Methods: women with vaginal discharges attending Kerbala Teaching Hospital for Gynocology and Obstetric for the period from January to March 2014 were enrolled in this study. High vaginal swabs were processed for the isolation and identification of Enterobacteriacae using standard microbiological techniques. Antimicrobials susceptibility testing was conducted for all of the isolated bacterial species. Results and discussion: swabs from 100 women were cultured. A total of 101 Enterobacteriacae isolates were recovered; 65 E coli, 23 P.mirabilis and 13 K.pneumoniae isolates. All K.pneumoniae isolates were resistant to ampicillin, amoxicillin-clavulanic acid and cefotaxime, whereas high resistance rates were detected to those antibiotics among E. coli (96.9%, 92.3% and 87.7%, respectively) and P. mirabilis (100%, 78.3% and 52.2, respectively). However, all isolates were susceptible to carbapenems (imipenem and meropenem) and low resistance rates detected to quinolones, aminoglycosides, lincosmaide and chloramphenicole. Resistance rates to aztreonam (ATM) were variable, high rates detected among E coli (69.2%) and K pneumoniae (61.5%) isolates, however, very low rates seen among P. mirabilis isolates (4.3%). Furthermore, high resistance rates were reported to tetracyclines and nitrofurantoin among E. coli (61.5% and 41.5% respectively), K. pneumoniae (69.2% and 100% respectively) and P. mirabilis (78.3% and 60.9% respectively). In addition, statistically significant differences were detected in the antibiotics susceptibility testing among the types of isolates to cefotaxime (p= 0.001), aztreonam (p= 0.000), cholramphenicole (p= 0.000), ciprofloxacin (p= 0.046), nitrofurantoin (p= 0.002) and amikacin (p= 0.007). Conclusions: Enterobacteriacae colonizing the cervico-vaginal epithelium are resistant to several important anitbiotics (multiple drug resistant) and thus may pose significant threat in transmission of drug resistant bacteria.
Keywords
Enterobactriacae; antibiotic susceptibility testing; high vaginal swabs

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