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Effect of Ficuscarica latex on mice organs and pheripheral human lymphocytes in vitro and in vivo | ||
| The Iraqi Journal of Veterinary Medicine (IJVM) | ||
| Article 2, Volume 39, Issue 2, 2015, Pages 1-5 PDF (0 K) | ||
| Authors | ||
| Khalil H. Ali Jeboori; Basim A.H. Al . Ghezy; Nahi Y. Yassin | ||
| Abstract | ||
| This study dealt with the toxic effect of the Ficuscarical latex experimentally in mice where the minimal lethal dose was calculated after intraperitoneal injection of Ficuscarical (30 mg/kg body weight), then four dilutions from the Ficuscarical material (1, 3, 4 and 5 mg/kg body weight) were tested. Four groups of mice, every group injected by one of these concentration (intraperitoneal by 0.2 ml/daily for 30 days), then the animals were euthanized. Gross lesions examination there were congestion, hemorrhage in kidney, liver, intestine and splenomegaly. The histopathological study also done for these formerly mentioned organs which showed hepatic and renal blood vessels hemorrhage and congestion, hydropic degeneration and necrosis of renal tubules and hepatocytes. There was dilatation of red pulp as well as accumulation of hemosidrin pigment and amyloid infiltration in spleen. The second aspect of experiment compare the effect of the FC latex with phytohemagglutinin and colchicin on human lymphocytes in vitro, which were cultured in ratio by doing 6 two fold dilutions of Ficuscarical material (31.25, 62.5, 125, 250, 500 and 1000 μg/ ml of culture medium). The resutlts showed that the 125 concentration up to high concentration of Ficuscarical had toxic effect on lymphocytes whereas, mitotic index were 0.13%, 0.2% for concentration of 62.5 and 31.25, respectively. Lymphoblasts mitotic index for two previous concentration were equal to 15%, 18%, respectively which was less than that recorded for phytohemagglutinin where the mitotic index was 0.6 and lymphoblasts mitotic index was 35%. In vitro study included comparison effect of different concentration of Ficuscarical latex on human lymphocyte during studying mitotic index, blastogenic index to phytohemagglutinin and colchicines. The Ficuscarical material does not act like colchicine for all concentration used in this study which were equal to same concentrations were used when compared with phytohemagglutinin. In conclusions, ficus latex had toxic effects on some organs of mice and was not inhibited blood lymphocytes growth, unlike colchicine and had stimulatory effects on human lymphocytes at low concentrations and toxic effect at high concentrations | ||
| Keywords | ||
| Ficuscarica latex; Mice organs; Human blood lymphocytes; In vitro | ||
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