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Aerobic biodegradation of phenol by Immobilized Pseudomonas sp. cells in two different bio-carrier matrices | ||
| Journal of Engineering | ||
| Article 1, Volume 22, Issue 4, 2016, Pages 68-78 | ||
| Authors | ||
| Zainab Ziad Ismail; Haneen Ahmed Khudhair | ||
| Abstract | ||
| Biotreatment using immobilized cells (IC) technology has proved to be the most promising and most economical approach for the removal of many toxic organic pollutants found in petroleum-refinery wastewater (PRW) such as phenol. This study was undertaken to evaluate the degradation of phenol by Pseudomonas cells individually immobilized in two different bio-carrier matrices including polyvinyl alcohol-guar gum (PVA-GG) and polyvinyl alcohol-agar agar (PVA-AA). Results of batch experiments revealed that complete removal of phenol was attained in the first cycle after 150 min using immobilized cells (IC) in both PVA-GG and PVA-AA. Additional cycles were confirmed to evaluate the validity of recycling beads of immobilized cells for phenol biodegradation. Results revealed that the phenol percentage removals were 95, 92, 86, and 84 % for second, third, fourth, and fifth cycles, respectively using Pseudomonas immobilized in PVA-GG beads. Whereby they were 96, 92, 90, and 84 % using Pseudomonas immobilized in PVA-AA beads for the same sequence of cycles. | ||
| Keywords | ||
| Immobilized cells; Pseudomonas; bio; carrier; wastewater; and phenol | ||
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