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A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice | ||
| Journal of the Faculty of Medicine | ||
| Article 1, Volume 51, Issue 2, 2009, Pages 220-222 | ||
| Authors | ||
| Shayma J. Ahmed; | ||
| Abstract | ||
| Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c). Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodium Dodecyl Sulfate(SDS), and urea without using Proteinase-K digestion or ultracentrifugation.Results: This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion.Conclusion: Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications | ||
| Keywords | ||
| DNA; Eukaryotes; extraction; mice; bone marrow; sperm; Hind III digestion | ||
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