- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
The Effect of Diode Laser 635nm on Mitochondrial Membrane Potential and Apoptosis Induction of CHO47cells line | ||
| Medical Journal of Babylon | ||
| Article 1, Volume 13, Issue 1, 2016, Pages 7-16 | ||
| Author | ||
| Rana Ayad Ghaleb | ||
| Abstract | ||
| Several studies suggest that low power laser irradiation is capable of affecting cellular processes under different conditions.In this study, the effect of low-power laser irradiation on CHO47 cells was investigated.The cells were irradiated with a CW, 635 nm Diode laser at an energy density ranging from 2.94 to 41.16 J/cm2 (power output, 100mW). The cells were grouped into: group1 control (not irradiated); group 2 exposed to 2.94 J/ cm² ; group 3 exposed to 20.58J/ cm²; and group 4 exposed to 41.16J/cm2. The mitochondrial membrane potential (MMP) of the irradiated cellsand apoptosis assaywas assessed at 96, 120 and 144 hours.In comparison with the control populations, a significant increase in mitochondrial membrane potential (MMP) of irradiated cells was observed. In addition, the irradiated cells showed a significant decrease in early, late apoptotic and dead cells in comparison with their respective controls.Based on the conditions of this study, we concluded that the low-level laser inhibit the apoptotic process and increase mitochondrial membrane potential (MMP). | ||
| Keywords | ||
| CHO cells; Laser irradiation; Apoptosis; mitochondrial membranepotential | ||
|
Statistics Article View: 76 PDF Download: 40 |
||