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The Influence of Helium- Neon Laser on Methicillin – Resistant Staphylococcus aureus using Photosensitizer(TBO) | ||
| journal of the college of basic education | ||
| Article 1, Volume 15, Issue 66, 2010, Pages 21-28 | ||
| Author | ||
| Ansam safaa husein | ||
| Abstract | ||
| The main objective of this study is to determine whether the use of He-Ne laser 632.8 nm, 7.5mW in combination with toluidine blue O (TBO) is an effective tool to eradicate MRSA, and the effect of laser light on the antibiotics sensitivity in vitro. Ten isolates were methicillin-resistant Staphylococcus aureus, after testing for their sensitivity to oxacillin 6µg/ml screening test and cefoxitin 30µg antibiotic disk. Following exposure to laser light with a wavelength of 632.8 nm in the presence of TBO at a concentration of 50µg/ml at various exposure times (5 min , 10 min , 15 min), highly significant statistical reduction in the viable count was achieved at the three exposure times , but 100% killing of cells was observed at exposure time of 15 min. While in the absence of the photo sensitizer there is no significant effect of the laser irradiation neither on the viable count at different exposure times observed, nor on changing their sensitivity to antimicrobial agents. | ||
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