Comparative immunizing status between repeated experimental infestation and injection of salivary glands extract of Hyalomma anatolicum anatolicum in sheep

A.F.M.AL-Taee,; L.Y.K.AL-Hyali,

	Comparative immunizing status between repeated experimental infestation and injection of salivary glands extract of Hyalomma anatolicum anatolicum in sheep
The Iraqi Journal of Veterinary Medicine (IJVM)
Article 2, Volume 38, Issue 1, 2014, Pages 1-8 PDF (0 K)
Authors
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Abstract
This study have been clarified the role of the repeated infestation with hard tick Hyalomma anatolican anatolicun and the use of native tick salivary glands extract in triggering the immunization of sheep. The study of native salivary glands using sodium dodocide sulphate polyacrilamide gel electrophoresis revealed that it consist of three bands (a, b and c) with molecular weight 67000, 65604, and 55531 Dalton respectively. For evaluation of immunization responses in sheep against hard tick including the nonspecific immunity which showed superiority in the absolute number phagositic cells in immunized group over that of the fifth group when determined from the first week till the end of the experiment 1294.03  30.91 ; 461.07± 10,88 and 1585.50  41.93 ; 1523,39  55,04 respectively. In the same order were the results of immunity which represented as hypersensitivity skin reactions, using skin there was higher intensive immediate type of it in the immunized group than that of the fifth infestation one after 30 minutes of injection with native salivary glands extract and were (77.2%,75.3%) respectively. Modified ELISA test was used for evaluation for measuring of humeral immunity that showed a significant elevation of absorbance intensity of the antibodies against salivary glands extract in the immunized group than the fifth infestation one 0.188, 0.139 respectively
Keywords
Immunization; salivary glands; Hyalomma anatolicum anatolicum; sheep

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