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ISOLATION AND PURIFICATION OF GLUTATHIONE S-TRANSFERASEFROM RAT LIVER | ||
| Journal of Al-Nahrain University - Science | ||
| Article 1, Volume 12, Issue 4, 2009, Pages 137-144 | ||
| Authors | ||
| Ayad H. Jassim; Zahraa F. Ameen; Essam F. A. Al-Jumaily | ||
| Abstract | ||
| AbstractGlutathione S-transferase (EC 2.5.1.18) was purified to homogeneity from rat liver. The enzymewas initially separated by a preparative anion exchange chromatographic step using DEAECellulose,then cation exchange chromatographic CM-Cellulose was used, followed by subsequentchromatography on Sephacryl S-200, the enzyme was purified about 419.88 fold with an 56.43%yield. The purified enzyme had a specific activity of 1250x10-3 unit/mg protein, indicated that theenzyme is momoner of Mr (50000) Daltons SDS gel electrophoresis studies. | ||
| Keywords | ||
| Glutathione; transferase; isolation; Purification; molecule weight; rat liver | ||
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