- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Broad-Spectrum Biofilm and Adhesion Inhibition by Levanase Purified from Enterobacter aerogenes Enterobacter aerogenes | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 26, Issue 2, 2015, Pages 1-8 | ||
| Author | ||
| Sahira Nsayef Muslim | ||
| Abstract | ||
| A novel strain of Enterobacter aerogenes isolated from agricultural by-products such as wheat, rice, and barely husks has the ability to produce intracellular levanase when grown on levan substrate. Levanase was purified to homogeneity with a recovery yield of 57.85% and 22.5 fold of purification by using ammonium sulfate at 40% saturation followed by ion exchange chromatography on Sepharose CL-6B column and gel filtration chromatography on Sephadex G-100 column. The purified levanase inhibited the biofilm formation ability in a dose-dependent manner for all Gram-negative and Gram-positive biofilm-forming bacteria and fungi(biofilm producers) and revealed antiadhesive activity toward all tested bacteria and fungi since the highest antiadhesive activity against Enterococcus faecalis was 93% and the lower antiadhesive rate was 38% against Staphylococcus aureus, thus the levanase has promising application as alternative antibiofilm agents to combat the growing number of multidrug resistant pathogen-associated infections, especially in situation where biofilms are involved. | ||
|
Statistics Article View: 37 PDF Download: 19 |
||