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Grape plantlets growth as indicators for tolerance of salt stress in vitro | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 7, Issue 3, 2015, Pages 1-11 | ||
| Authors | ||
| Muslim A.A. Abdulhussein; Ahmed HamzaHashim | ||
| Abstract | ||
| An experiment was carried out test salt tolerance of three grape (Vitisvinifera L.) seedless cultivars ( Crimson seedless , Princess and Summer Royal) in vitro , according to effects of NaCl on plantlets growth indicators for the selection of the most salt-tolerant cultivar . Plantlets were cultured in liquid MS medium provided with 5 concentrations of NaCl (0, 50, 100 .150 and 200) mM for 21 days in culture room. At the end of experiment ,growth indicatars plantlets survival percentage, plantlets damage index , number of leaves , plantlets dry weight, and root dry weight system were calculated .Adding sodium chloride to the growth medium caused reducing the plantlets survival percentage, vegetative and root growth indicators ( average number of leaves , plantlets dry weight, and root dry weight system,) and increasing the degree of plantlets damage with increasing the concentration of sodium chloride salt in growth medium up to 200 mM. There were a significant differences in response of grape cultivars the addition of sodium chloride salt to growth medium. Results showed that Crimson seedlessand Summer Royal were more salt tolerant compared with Princess. No significant differences were noted between Crimson seedless and Summer Royal in survival percentage , degree of plantlets damage and root dry weight .There were a significant differences between interaction treatments in which Crimson seedless plantlets grown in freeNaClmedium gave higher values in the Survival percentage of plantlets, average number of leaves , plantlets dry weight, and root dry weight, and less degree damaged, while the lowest values were in the plantlets of interaction treatments between (200 mM and Crimson seedless , Princess and Summer Royal) in the survival percentage of plantlets, number of leaves , shootdry weight , root dry weight . | ||
| Keywords | ||
| grape plantlets; invitro; Salt Stress | ||
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