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Study The effect of different dilution to the semen samples on DNA integrity in the sperm of Awassi Rams | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 7, Issue 4, 2015, Pages 66-72 | ||
| Authors | ||
| Hiam AL-Husseiny; Tahreer Mohammed; Ismael KadhumAjam | ||
| Abstract | ||
| This study was conducted at College of Agriculture / AL-Qasim Green Universityfor the period from 1/9/2014 and up to 28/5/2015 on Al-awassi rams trained to semen collection and this study aimed to determine the impact of additive Nutrient extender added to the semen samples on DNA Integrity of spermatocyte in Al-awassi rams using "Comet Assay". Two types of common extender were in this study used for semen dilution and they are( Tris dilute and milk dilute added to them egg yolk) and compare , diluted samples with fresh samples through the assessment of DNA integrity of spermatocyte. The results of current study showed the following :Significant effect (P ≤ 0.05)of diluted type on the tail length of a comet, where fresh samples showed high level of DNA damaged (% 9.45 ± 0.46 ) compared to the diluted samples in Tris diluted (% 4.68 ± 0.18) and followed by the milk diluted (% 6.21 ± 0.20 ) .Type of extender showed significant effect (P ± 0.05) on the percentage of DNA in the tail and the tail moment where the milk diluted samples showed medium level of the damaged DNA ( % 6.05 ±0.28 ) and ( %0.62 ± 0.52 ) respectively.This study concluded that Tris dilute concentration(4.2%) was the best type in the DNA integrity compared to Milk dilute and control samples. | ||
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