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Bacteriological comparative of some genus of Enterobacteriaceae isolated from Hospital birth rooms in Baquba city | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 11, Issue 1, 2015, Pages 10-23 | ||
| Authors | ||
| Duaa Adnan Kadhum Alutbi; Hadi Rahman Rasheed Al-Taai; Adnan Namma Al Azawy | ||
| Abstract | ||
| The study included isolation some genus belong to Enterobacteriaceae from Eat birth, clinical and environmental sources in some hospitals in Baquba city.The results showed that 40 isolates belong to Enterobacteriaceae .15 isolates (37.5%) E.coli, 9 isolates (22.5%) P.mirabilis, 6 isolates (15%) K.pneumoniae by using biochemical diagnosed tests and confirm the diagnosis by using Api 20E system. The sensitivity of the isolates toward 14 antibiotics were tested . The results showed a variance as far as their resistance to these antibiotics. Isolates of E.coli showed highest resistance rate % 93.3 for Piperacilline, while isolates of Enterobacter spp resistan with 90% for Cefixime and Tobramycin. P.mirabilis higher resistance to Ampicillin by100% , while K.pneumoniae showed resistance to Ampicillin and Cefiximewithpercentage 83.33%. Imipenem is the most effective antibiotic by(100%). The minimum inhibitory concentrations (MIC) for 5 antibiotics, that Amoxicillin,Cefotaxime,Ciprofloxacin, Streptomycin,Nalidixic acid were determined. The resutes indicated that MIC values of these antibiotics ranged between (64-1024>) , (32-1024>), (2-1024) ,(128-1024) ,(8-1924>) µgml, respectively. The combination of antibiotics among Ciprofloxacin ,Streptomycin and Cefotaxime by combination (1:0.5,1:1,1:2,1:3). The results showed that the combination ratio (1:3) is considered the best was among other ratios. The combination of Cefotaxime and Ciprofloxacin showed synergistic effects by (96.29%), when combination , Streptomycin with Cefotaxime showed Synergy rate of (81.48%) . | ||
| Keywords | ||
| Enterobacteriacea; Antibiotics; combination | ||
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