Polymorphism of Glutathione-S -Transferase P1 gene in Breast Cancer patients in Baghdad /Iraq

Qassim Muftin, Najlaa; H. N. AL-Rubai, Salwa; Y. Yaseen, Nahi

	Polymorphism of Glutathione-S -Transferase P1 gene in Breast Cancer patients in Baghdad /Iraq
Iraqi Journal of Cancer and Medical Genetics
Article 1, Volume 8, Issue 2, 2015, Pages 117-122
Authors
Najlaa Qassim Muftin; Salwa H. N. AL-Rubai; Nahi Y. Yaseen
Abstract
Glutathion S-transferase enzymes are detoxifying enzymes – phase II enzymes, which play an important role in protect- ing cells from damage caused by endogenous and exogenous compounds and subsequently, are crucial in protecting the DNA. The aim of this study is to evaluate polymorphism of Glutathion S-transferase P1 (GSTP1) in Iraqi women breast cancer. Blood samples were collected from 65 Iraqi women diagnosed as breast cancer patients who attended to Oncology Teaching Hospital and Al-Yarmouk Teaching Hospital. In addition to 59 healthy women as control group. Genomic DNA was extracted then genotyping was performed by restriction fragment length polymorphism- polymerase chain reaction (RFLP- PCR) for GSTP1 gene. The frequencies of GSTP1 polymorphism (Ile105Val) in the breast cancer group were: 41.54% for wild type (Ile/Ile), 49.23% for heterozygote (Ile/Val) and 9.23% for homozygote mutant (Val/Val), while in control group was 57.63 for wild (Ile/Ile), 42.37(Ile/Val) heterozygote and 0.00 %(Val/Val) homozygote. The results show there were statistically signi cant differences in the distribution of (Val/Val) genotype and Val allele between patients and controls groups (p<0.05), (OR= 16.3091; 95% CI= 0.8798 to 302.3326) and (OR= 1.9380; 95% CI= 1.0274 to 3.6556) respectively . This study showed that GSTP1 (Val/ Val) genotype may be associated with an increased risk for breast cancer among Iraqi women.
Keywords
glutathione S; transferase; Breast Cancer; GSTP; restriction fragment length polymorphism

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