Biological study of Proteus mirabilis isolated from different clinical sources in AL-Mqdadia city

A. Mahmood Al- Rajab, Ibrahim; R. Rasheed Al-Taai, Hadi; N. Al- Azawy, Adnan

	Biological study of Proteus mirabilis isolated from different clinical sources in AL-Mqdadia city
Diyala Journal For Pure Science
Article 1, Volume 11, Issue 2, 2015, Pages 42-60
Authors
Ibrahim A. Mahmood Al- Rajab; Hadi R. Rasheed Al-Taai; Adnan N. Al- Azawy
Abstract
Two hundred twenty five samples were included (100 samples – Otitis media, 92 burning infection and 33 wound infection) of the patient in Al-Muqdadia – Diyala Governorate for the period November 2012- March, 2013. The results of bacteriological testing on blood agar, MacConky agar, and diagnose by microscope test, biochemical tests and certainty test by the use of api 20 E showed that 37 Isolates belong to P. mirabilis. Virulence factors were investigated by the isolates understudy. The study reveals that isolates were able to produce Haemolycine and Urease enzyme and the formation of Swarming phenomenon with rate 100%. Concerning Biofilm the study reveals that the isolates were able to form Biofilm, by using ELSA method 72.97%, Tube method 67.56% and Congo red method 5.4%. The ability to produce β-lactamase enzymes were investigated. The results showed that P. mirabilis were able to produce β-lactamase enzymes with 91.89%, while production of Extended Spectrum β-lactamase 56.75%. This bacteria produced Metalo β-lactamase enzymes 13.51%. The results induct that Proteus mirabilis had resistance to the antibiotics: Nitrofuration, Cefotaxime, Ampicillin, Cefalothin, Ceftazidime, Trimethoprim with the rates 100%, 100%, 100%, 91.9%, 97.3% and 91.9% respectively. Whereas the ability of the resistance to other antibiotics was less. They were: Tobramycin, Clavulanic acid, Amoxicillin, Gentamycin 81%, 81% and 83.8% respectively. The isolates were more sensitive to Amikacin 43.2%. The best two antibiotics to deal with P. mirabilis were Imipenem and Ciprofloxacin with resistance rate 16.2% and 10.8% respectively. The results revealed isolates with multi-resistance type; these isolates were divided into two groups depending on the number of antibiotics which isolates resisted. The first group included (9) isolates 24.32% which showed resistance to (4- 8) antibiotics whereas the second group which included (28) isolates 75.67% which resisted to (9- 12) antibiotics. The results showed that the second group was the supporter.
Keywords
Proteus mirabilis; virulence factors; antibiotic

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