Bacteriological and Genetic study of some genus for bacterial gram-negative resistant to β-lactam , isolated from urinary tract infections

Abod Farhan Aldulemia, Abass; Rahman Rasheed Al-Taai, Hadi; Khudhair Abass Alniaamy, Mohammed

	Bacteriological and Genetic study of some genus for bacterial gram-negative resistant to β-lactam , isolated from urinary tract infections
Diyala Journal For Pure Science
Article 1, Volume 11, Issue 3, 2015, Pages 66-76
Authors
Abass Abod Farhan Aldulemia; Hadi Rahman Rasheed Al-Taai; Mohammed khudhair Abass Alniaamy
Abstract
This study included collection 300 samples from patients suffering from urinary tract infection from Baaquba Teaching Hospital and Al –Batool Hospital in Baaquba city for the period from 1/09/2013 to 1/01/2014. The results refer that 66 isolates are belonging to bacteria of Gram negative (57.4%), 25 (37.78 %) Escherichia coli , 22(33.33%) Proteus mirabilis ,by using diagnostic phenotypic ,biochemical tests and confirm the diagnosis using regular API20E. The production of -lactamase by Eschrichia coli and Proteus mirabilis was (60%) , (40.9%) respectively, also the isolated had the ability to produce the Extendended spectrum β-Lactamase enzyme by using disc Approximation ,The production from each ofEschrichia coli and Proteus mirabilis (12%), (31.8 %)respectively. The results of Metalloβ-Lactamase by using the Imp-EDTA combination inducted that E.coli and P.mirabilis were (12%) and (13.6%) respectively. The results of molecular detection of ESBL genes (bla TEM and bla SHV) by using PCR technique ,(9) samples from (10) total , divided into 3(100%) E.coli and 6(85.7%) P.mirabilis were harboring bla TEM gene based on the presence of 950 bp bands in 1% agarose gel. while results detect that the isolates of Escherichia coli and Proteus mirabilis not harboring bla SHV gene.
Keywords
urinary tract infections; lactamase; bla TEM and bla SHV; PCR; bla TEM

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