Study the Efficiency of Some Types of Bacteria on Producing of Biofuels

Sattar Abdul Jabbar, Abdul; A. Khadom, Anees; Neama Al Azawy, Adnan

	Study the Efficiency of Some Types of Bacteria on Producing of Biofuels
Diyala Journal For Pure Science
Article 1, Volume 11, Issue 4, 2015, Pages 37-44
Authors
Abdul Sattar Abdul Jabbar; Anees A. Khadom; Adnan Neama Al Azawy
Abstract
The study included isolative and diagnose of some types of anaerobic and aerobic bacteria capable of decomposing and fermentation of cellulose. Isolation and diagnose the bacteria Clostridium phytofermentous from 10 agricultural soil samples, was 50 percent of the total 50 colony, the isolation of bacteria Eschrichia coli from 15 waste water samples which represented 40% of the total 75 colony and isolated the bacteria Pseudomonas aeruginosa from 15 normal soil samples, and was 53% of the total 75 colony. The ability of studied bacteria on the production of cellulose decomposing enzymes was evaluated using absorbance method. Higher activity obtained via Clostridium phytofermentous bacteria with maximum value of 2.52 IU / mL, while Pseudomonas aeruginosa gives 2.21 IU / mL and Eschrichia coli unit gives 2.09 IU / mL. The results show the effect of some environmental conditions on the effectiveness of the production of the enzyme cellulose. Optimum temperature range was 35-40 0C and pH ranges of 7-9. The concentration of ethyl Alcohol was measured using Titration method. Maximum concentration of alcohol produced by bacteria Eschrichia coli, which reached 8.28 g/liter, while the bacteria Pseudomonas aeruginosa reached 7.86 g/liter, and Clostridium phytofermentous reached 6.62 g/L.
Keywords
cellulose; enzyme; Enzyme activity; Biofuel

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