Bacteriological Comparison Between Pseudomonas aeruginosa and Klebsiella pneumoniae Isolated from Different Infectious Sources

R. Rasheed AL-Taai, Hadi

	Bacteriological Comparison Between Pseudomonas aeruginosa and Klebsiella pneumoniae Isolated from Different Infectious Sources
Diyala Journal For Pure Science
Article 1, Volume 12, Issue 1, 2016, Pages 162-182
Author
Hadi R. Rasheed AL-Taai
Abstract
Three hundred samples collected from different clinical sources(burns, wounds, urine,) have been isolated and identified, including 56 Isolates of Pseudomonas aeruginosa and 18 Isolates of Klebsiella pneumoniae then confirmed diagnosis by using regular api20E, api-20NE and VITEK 2 system. Results of antimicrobial susceptibility showed that all isolates were resistant to Ampicillin, Amoxicillin, Cephalexin, Nitrofurans and Co-Trimoxazole with ratio 100%, while all isolates resistant imipenem with 0%. Results of determination dominant pattern of multiple resistance to antibiotics by Pseudomonas aeruginosa was AM - AMX - FT- CL - SXT- Cb -CTX-CTR-PRL-ATM –CN- AK- CIP , wheals the pattern of resistance by Klebsiella pneumoniae was Amp –AMX- SXT-FT - CL - Cb - CTX - CTR—AK-CIP- CN - ATM. The results showed that 30 isolates of the total (56) of P. aeruginosa could produce β-Lactamase with percentage (53.5%), also 14(77.7%) isolates of the total (18) of Klebsiella pneumoniae gave positive result. K. pneumoniae have ability to tolerant heavy metals like (copper, zinc, mercury, cobalt ) with 38.8%, 50%,16.6% , 38.8% respectively, while P. aeruginosa tolerant same heavy metals with percentage ( 17.8%, 41%, 16.6%, 25%) respectively. Additionally, results of plasmid profile presented that all isolates of Klebsiella pneumoniae contained tow bands of plasmid vary in size, some isolates contained the Mega Plasmid, wheals found that all isolates of Pseudomonas aeruginosa contained single plasmid and all sizes of plasmids were relatively close. Analysis of conjugation results showed 12(85.7%) transconjugant of Klebsiella pneumoniae and 25(83.3) transconjugant of Pseudomonas aeruginosa revised character β-Lactamase production. Results showed the highest percentage transfer of antibiotic resistance was to Ampicicillin, Amoxicillin and Carbencillin with 100%, while the lowest percentage of resistance was to Gentamicin by 35.7% in both Klebsiella pneumoniae and Pseudomonas aeruginosa. Results showed that the highest proportion of transfer of heavy metals to zinc metal reaching to ratio 100%, whilst less transmission was mercury metal with ratio 16.6 %.
Keywords
pseudomonas aeruginosa; Klebsiella pneumoniae; Antibiotics; conjugation

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