The Effect of Thymus vulgaris Extracts on Enterotoxins (A-D) Production by Staphylococcus aureus Isolated from Urinary Tract Infection

Harby Kadhim, Akbal; Abead Hassoni, Adil; Jawad Kadhim, Iman

	The Effect of Thymus vulgaris Extracts on Enterotoxins (A-D) Production by Staphylococcus aureus Isolated from Urinary Tract Infection
Diyala Journal For Pure Science
Article 1, Volume 12, Issue 2, 2016, Pages 1-17
Authors
Akbal Harby Kadhim; Adil Abead Hassoni; Iman Jawad Kadhim
Abstract
One hundred and eighty five urine samples were collected eight isolates (4.3%) were obtained and diagnosed as Staphylococcus aureus. The identification of all S. aureus isolates was confirmed by conventional biochemical tests. Among 8 isolates, 5 (62.5%) S. aureus isolates were found to be enterotoxigenic . Approximately most of isolates produced at least two types of staphylococcal enterotoxins ( SEs). These enterotoxins may be important in the physiopathology of urinary tract infection . The production of staphylococcal enterotoxins (A–D) in the presence or absence of Thymus vulgaris the crude extracts (aqueous and alcoholic ) were was estimated in microtiter plates using a reversed passive latex agglutination (SET-RPLA) kit . The extracts reduced enterotoxin production at subminimal inhibitory concentrations compared with the control. At 400 μg/ml , total enterotoxin inhibition was observed for enterotoxin C production, whereas production of enterotoxins A, B, and D were totally eliminated at 800 μg/ml . The results show that the aqueous and alcoholic extracts from the leaves of T. vulgaris significantly decreased, in a dose-dependent manner, the production of SEA, SEB,SEC and SED by S. aureus .
Keywords
Staphylococcus aureus; Staphylococcal enterotoxins; Thymus vulgaris; urinary tract infection

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