Study the Effect of Different Concentrations of Kerosene in Some Physiological Activities in Oscillatoria Chlorina

A. Salim, Ayman; M. Sultan, Ali; Salman Khamis, Hamid

	Study the Effect of Different Concentrations of Kerosene in Some Physiological Activities in Oscillatoria Chlorina
Diyala Journal For Pure Science
Article 1, Volume 12, Issue 2, 2016, Pages 86-102
Authors
Ayman A. Salim; Ali M. Sultan; Hamid Salman Khamis
Abstract
The effect of different concentrations of kerosene had been examined upon Oscillatoria chlorina, The optical density had been depended as measure for growth as well as chlorophyll concentration, Phycocyanin pigment and protein concentration as an indicator of kerosene effects on Oscillatoria chlorina, in addition to morphological changes. The optical density of growth in the control sample was (0.43), while it was (0.36) at 0.25% kerosene concentration, and the growth inhibited at ≥ 1%. At 0.25% kerosene, the chlorophyll pigment concentration in the 8th day was half of that in control sample in the same day. Also that species tolerates the effect of this concentration of kerosene As the chlorophyll concentration record (0.72) mg/ml at the end of growth period. while influence of 0.5% kerosene, the chlorophyll pigment concentration was decline to 0.42 mg/ml, and totally reduced under 1% & 2% of kerosene concentration. The protein concentration also reduced under the effect of 0.25% & 0.5%of kerosene and totally reduced at 1% & 2% of kerosene. Some morphological changes noted under the effect of kerosene as thickening of cell wall and increase in cellular diameter. As well as the absorption spectrum infrared by FTIR show that the Oscillatoria chlorina consume sy & asy methyl group (CH3) at (2870 cm-1) & (2950 cm-1), and absence of bending bundle at the (1430 cm-1) & (1380 cm-1) which were present in the control sample.
Keywords
Oscillatoria chlorine; cyanobacteria; kerosene; Oscillatoria chlorina

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