Stimulating Growth Rates and Doubling Time of Green Alga Scenedesmus. quadricauda by Using Different Culture Medium

M.A Alsalman, Ibrahim; M Ibrahim, Thaer

	Stimulating Growth Rates and Doubling Time of Green Alga Scenedesmus. quadricauda by Using Different Culture Medium
Diyala Journal For Pure Science
Article 1, Volume 12, Issue 3, 2016, Pages 78-93
Authors
Ibrahim M.A Alsalman; Thaer M Ibrahim
Abstract
The current study included highlighting on the ability of stimulating of quality of Culture medium in growth rates and doubling time of individuals of green Scenedesmus.quadricauda under laboratory conditions. It used three agricultural media, Chu10, BG11 and Chu13 axis, as showing in methods ,and (Chu10) considered as (control) for the purpose of comparison. Absorbance standards under wavelengths adopted (540-650) nm respectively and promised cells in a manner sector browser using counting pellets slice to draw a growth curve, to calculate growth rates and determine the time replication, by conducting daily measurements and up to 25 days. The results showed that type and contents of culture medium have a clear impact on the absorbance values, number of cells and the stages of the growth curve of studied alga, and when relying on the number of cell, growth curve showed a clear difference in the extension phases of growth (logarithmic, stability and death) when development in the three used media, as it was the end of the logarithmic phase at days 5, 6 and 9 of Chu10, Chu13, BG11, and (4.4 and 2.39 and 5.37) × 106 cells / ml respectively. The phase stability continued growth in Chu10, of the day 6 to 25 (the end of the experiment), while in BG11and Chu13 was 5-12 and 9-12 days respectively, with the fluctuation in values and a significant increase in the number of cells in BG11 Chu13 media compared with Chu10, as the highest value recorded during the phase 5.25, 4.2 and 2.4 × 106 cells / ml respectively. Also the results of absorbance showed that, the values at wavelengths between them 540 – 650nm recording close to each other, and agree with the cells number, with superiority of BG11and Chu13 as well as the characteristic values of the oscillation in Chu10 media. It is worth mentioning that, the absorbance values of 540 and 650 nm, did not show death phase or (sharp decrease) in spite of the declining number of cells, paraphrase affected of its values by the components of the media and the remains of dead cells and not only the number of live and active cells. As regarding the growth rate and time replication of studied alga, results showed clear differences between the values of (Chu10) axis and the two other media during 25 days. As it recorded the highest growth rate in Chu13 reached .2.9 and less doubling time of 9.9 hours, and in BG11, was 2.22 and 511.7 hour while they were in Chu10, 1.9 and 47.9 hours, respectively.
Keywords
Culture medium; Algae; Scenedesmus quadricauda; growth rate; doubling time

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