The Bactericidal Activity of Gamm ,Beta and of Semiconductor Laser Irradiation Effect on Klebsiella pneumoniae  (in vitro )

. S. Abd, Raad; H. Mahdi, Khalid; R. Mohammed, Nebras; S. Sabaa, Hanaa

	The Bactericidal Activity of Gamm ,Beta and of Semiconductor Laser Irradiation Effect on Klebsiella pneumoniae (in vitro )
Magazine of Al-Kufa University for Biology
Article 1, Volume 0, Issue 0, 2017, Pages 218-222
Authors
Raad . S. Abd; Khalid H. Mahdi; Nebras R. Mohammed; Hanaa S. sabaa
Abstract
This work evaluated the effect of gamma , beta and Semiconductor laser irradiation on Klebsiella pneumoniae isolated from sputum . The experiment included a control and 4 doses of each gamma and beta irradiation ,which ranged(1.317×10-4 -0.320µSv) and (10.573-96.950µSv) respectively. The total effect of gamma and beta irradiation on K. pneumoniae viability was abrogated at (96.915177 & 63.100 µSv) by 137Cs and 90Sr respectively, the percentage of killing was highly (81%) and (85%) respectively and the viable cells was fewer than control . This work evaluated also the effect of Semiconductor laser on K pneumoniae. The experiment included a control and triplicate of K. pneumoniae exposed to Semiconductor laser in power 5 mW , in Wavelength 650 nm. The effect of Semiconductor laser on the viability of K. pneumoniae was counted and percentage of killing was counted , the number of viable cells of K. pneumoniae was fewer than control (without exposure to this laser) , but percentage of killing was higher than control . Semiconductor laser was efficient to killing K. pneumoniae , and the Gamma and Beta irradiation is efficient to killing K. pneumoniae that cause many infection to human and may be cause death .
Keywords
Klebsiella pneumonia; Semiconductor laser; Gamma and Beta irradiation

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