Study The Interaction between LH, FSH, and TSH with New Synthesized Magnetic Nanoparticles Coated with Dextran

Fadhel Al-Kazazz, Fatin; Kadhem Al-Hakeim, Hussain; Khdair Al-Aobaid, Habiba

	Study The Interaction between LH, FSH, and TSH with New Synthesized Magnetic Nanoparticles Coated with Dextran
Medical Journal of Babylon
Article 1, Volume 13, Issue 2, 2016, Pages 0-0
Authors
Fatin Fadhel Al-Kazazz; Hussain Kadhem Al-Hakeim; Habiba Khdair Al-Aobaid
Abstract
Nanoparticle (NP) was used in vivo and in vitro in contact with the body fluids that contain different types of proteins. The proteins have the ability to adsorb on NPs surface, and alter its activity due to the modification of the secondary and tertiary structures. In this study, the interaction between coated magnetic nanoparticles (MNP@Dextran) and the hormones (LH, FSH, and TSH) were studied in details. The possible change in the secondary and tertiary structure of these parameters is discussed. The (MNPs) were prepared using co-precipitation method of Fe3O4 followed by reacted these MNPs with Dextran under an optimum conditions to produce coated MNP@Dextran. Different concentrations of each hormone were incubated with a known amount of MNP@Dextran and the quantity of the hormones adsorbed was calculated. The experiments were repeated at different temperatures to calculate the thermodynamic parameters. Secondary structures of hormones were examined using circular dichroism (CD).Tertiary structures of protein were measured using fluoroscopy technique.The adsorption process obeyed Freundlich adsorption isotherm indicating heterogeneity of the MNP@Dextran surface,and it associated with the changing in the secondary and tertiary structure of hormone after adsorption on MNP@Dextran.
Keywords
Magnetic nanoparticles; protein adsorption; FSH; TSH and protein structure

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